Field Synthesis Light-Sheet Microscopy

One approach for high-resolution light-sheet imaging is to use a superposition of propagation invariant beams as your illumination source, as is done in Lattice Light-Sheet Microscopy (LLSM). Nonetheless, because such illumination beams require projecting a complex amplitude and phase profile onto the back pupil of an illumination objective with a spatial light modulator, they often require expert assembly and operation. To overcome such challenges, we will develop an easy-to-assemble Field Synthesis light-sheet microscope. In Field Synthesis, a focused line is scanned over an appropriately designed binary pupil mask with a galvanometer during a single camera exposure. Thus, all that is needed for Field Synthesis is a galvanometer, a phase mask, and a 4f telescope. Importantly, Field Synthesis is statistically indistinguishable to LLSM in terms of resolution and photobleaching.