Train the Trainers
This inaugural workshop focused on outreach to major microscope core facilities across the nation to collect feedback. Consequently, we had the following personnel participate and help refine our dissemination strategies.
Adriana Paulucci, PhD.
Microscopy Core Director at the Department of Genetics, MD Anderson Cancer Center.Asier Marcos Vidal, PhD.
Imaging Specialist at the W. M. Keck Microscopy Facility, Whitehead Institute, MIT.Brandon Scott, PhD.
Assistant Professor at South Dakota Mines.Christina Pyrgaki, PhD.
Head of the Advanced Light Microscopy Innovation Lab at Memorial Sloan Kettering Cancer Center.Eva Lucia de la Serna, PhD.
Advanced Microscopy Fellow at the Harvard Medical School.Evolene Premillieu, PhD.
Light Sheet Specialist at the BioFrontiers Institute at University of Colorado Boulder.Gary Laevsky, PhD.
Director of Confocal Imaging Facility within the Department of Molecular Biology at Princeton University.Kersi Pestonjamasp, PhD.
Manager of Resource for Microscopy within Moores Cancer Center at University of California San Diego.Michelle Itano, PhD.
Director of Neuroscience Microscopy Core at University of North Carolina Chapel Hill.Nicolas Denans, PhD.
Imaging Application Scientist at HHMI Janelia Research Campus.Owen Puls, PhD
Bioimage Data Analyst at HHMI Janelia Research Campus.Priyam Bannerjee, PhD.
Senior Research Support Specialist (Optical Microscopy) for the Bioimaging Research Center at Rockefeller University.Rachel Lee, PhD.
Bioimage Data Analyst at HHMI Janelia Research Campus.Tse-Luen Wee, PhD.
Director of Microscopy Shared Resource at Cold Spring Harbor Laboratory.Zulin Yu, PhD.
Head of Light Microscopy at Stowers Institute for Medical Research.
September 23rd to October 5th, 2024
UT Southwestern Medical Center
Dallas, TX
This intensive two-week workshop was designed to equip staff in select academic microscopy core facilities to adopt CCSA’s technologies for imaging and analyzing cellular signal transduction and transfer the know-how to their user groups. The workshop featured in-depth interactive lectures on the background of the technologies and extensive hands-on lab sessions that demonstrate the capabilities and challenges of the current states of the technologies. The goal of this program was to open a long-term bi-directional dialogue between technology developers, disseminators, and appliers that will empower the cell biology research community.
Program
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List of lectures included in zipped folder linked above:
Multispectral imaging
Traction force microscopy
Introduction to the software suite
Introduction to light sheet microscopy
Imaging cells in different environments
Advanced segmentation approaches
Data visualization
Oblique plane microscopy (OPM)
Introduction to imaging translocation biosensors in 3D
Post-processing of OPM data
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Lectures have been grouped into PDFs by days at link to zipped folder above and are listed below.
Day 1: FRET, allosensors, SPECTR
Day 2: LOV-based
Day 2 & 3: Loopology RapR
Day 3: FRET Analysis
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Image Variables in Shape Invariant Space - part 1
Image Variables in Shape Invariant Space - part 2
uUnwrap3D
u-quantify
Cellular Harmonics - u-Signal3D
Particle Tracking
Trajectory Diffusion Analysis
Multivariate Particle Analysis Colocalization
Multivariate Image Processing
Granger Causality
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Overview
Multiscale Microscopy
Smart Microscopy
Introduction to Navigate
Modern Data Handling
Statistical Analysis - Zebrafish
Cyclic Profiling of tissues in 2D
Cyclic Profiling of thick tissue
Tissue Clearing
Segmentation
uSegment3D
Cancer across Scales - Bio-Discoveries