Train the Trainers

This inaugural workshop focused on outreach to major microscope core facilities across the nation to collect feedback. Consequently, we had the following personnel participate and help refine our dissemination strategies.

September 23rd to October 5th, 2024

UT Southwestern Medical Center
Dallas, TX

This intensive two-week workshop was designed to equip staff in select academic microscopy core facilities to adopt CCSA’s technologies for imaging and analyzing cellular signal transduction and transfer the know-how to their user groups. The workshop featured in-depth interactive lectures on the background of the technologies and extensive hands-on lab sessions that demonstrate the capabilities and challenges of the current states of the technologies. The goal of this program was to open a long-term bi-directional dialogue between technology developers, disseminators, and appliers that will empower the cell biology research community.

Program

  • All lecture PDFs

    List of lectures included in zipped folder linked above:

    1. Multispectral imaging

    2. Traction force microscopy

    3. Introduction to the software suite

    4. Introduction to light sheet microscopy

    5. Imaging cells in different environments

    6. Advanced segmentation approaches

    7. Data visualization

    8. Oblique plane microscopy (OPM)

    9. Introduction to imaging translocation biosensors in 3D

    10. Post-processing of OPM data

  • All lecture PDFs

    Lectures have been grouped into PDFs by days at link to zipped folder above and are listed below.

    Day 1: FRET, allosensors, SPECTR

    Day 2: LOV-based

    Day 2 & 3: Loopology RapR

    Day 3: FRET Analysis

  • All lecture PDFs

    1. Image Variables in Shape Invariant Space - part 1

    2. Image Variables in Shape Invariant Space - part 2

    3. uUnwrap3D

    4. u-quantify

    5. Cellular Harmonics - u-Signal3D

    6. Particle Tracking

    7. Trajectory Diffusion Analysis

    8. Multivariate Particle Analysis Colocalization

    9. Multivariate Image Processing

    10. Granger Causality

  • All lecture PDFs

    1. Overview

    2. Multiscale Microscopy

    3. Smart Microscopy

    4. Introduction to Navigate

    5. Modern Data Handling

    6. Statistical Analysis - Zebrafish

    7. Cyclic Profiling of tissues in 2D

    8. Cyclic Profiling of thick tissue

    9. Tissue Clearing

    10. Segmentation

    11. uSegment3D

    12. Cancer across Scales - Bio-Discoveries